痂囊腔菌属PKS基因特性分析.docx

Characterizationofpo1.ykctidcsynthasegenesfromE1.sinoespp.和囊腔MPKS嘱因特性分析目的：得到花生疮痂病的的PKS域因，明确其PKS的结构域，证明由素的合成受到PKS的网控.方法：I使用简并引物对其胡君合iKS保守区进行扩增，通过染色体步格，泅得其全长.2对荻得的全长翻译后进行宽基酸的进化树分析.确定其种类3对基因表达进行研究讨论：Materia1.andmethodsFunga1.strainsandgrowthconditions尾胞(Genebanktiff).柑橘(Genebimk己有，他葡,花生Iso1.ationoftheketovnthase(KS)domainusingdegenerateprimers采用简并引物扩增PKS的KS保守区域.步骤方法Identificationofpartia1.PKSsequencesKS保守区域熨基取序列对比(尾他.柑橘.匍葡,花生.黄曲褥毒索,爆刀菌素)Genomewa1.king获得权因全长，标记各个PKS域分布及与其他相似的比较SAPKSPhy1.ogeneticana1.ysisofPKSsequences进化树选择不同类型的PKS基因类型1.2.3),选择还原鞭及非还原型.(参考2003)选择还原型和非还原型两种.Expressionana1.ysesofPKSgenes测定各个域的表达量及整个基因的衣达量，明确该基因的表达是否与光照有相关性，或者存在其他相关联基因共同调节次生代谢产物.>SAPKSEkafage1.gsigtssr1.vtayea1.eesgyvpnrtpstk1.iirigtfygqtsddwreinaaenVdtyfitggvrafakirixyyfkfsgpsfsv,Baaiqi.actsi.wagk'dtacagg1.nv1.tnpdifsg1.skgqf1.sktgscktydndadgycrgdgcgsvi1.kryedaiadkdni1.Gci1.gaatnhsaeavsitiiphagaqef1.fkkv1.adagidahqidyvemhgtgtiii> (E1.sinocIawocttiiMsnv1.1.1.gdqtadqsp1.1.rk1.v1.rrnda1.vttfheqvsva1.rdevsk1.parqrqdipsf1.k1.n1.vd1.yyQGDKRI.Pi1.estfvaitq1.ghyigyfsenpdr1.pyaantrv1.g1.ctgs1.aatvivsaksise1.vs1.veVriafragacvdaakrv1.qqpgeekepwstivmn1.sekdaqka1.defhsstgtsgpsrayIsaistmavtIsgppsttqk1.fseszxd1.stknrvsipiyapyhaah1.hserd1.qriipdssra1.1.kkyqpht1.1.hssagEC1.VADNTCD1.MSI.AI.VDFI.REPVRWDRM.EESISQ11ATPKAPAKIFTIGVSNVANSMAAA1.KSGQATVsitdhtswivpddynstrgrtqndkiaivgmsgrfpsaasvda1.we1.1.ekg1.dvhrkipaDrfdadahcDpsgksknkshtpygcfidepg1.fdprffnmspreazxqtdpmgr1.a1.vtayezx1.eesgyvpnrtpstk1.hR1.GTFYGQrSDDwREINAAENVDrYFrrGGVRAFAPGRINYYFKFSGPSES1.HHaAiq1.acts1.WGDCDTACAGG1.NV1.TNPDISG1.SKGQ1.SKTGSCKTYDNDADGYCRGDGCGSVI1.KRyedaiadkdnI1.GCI1.GAATNHSAEAVSrrHPHAGNQEF1.FKKV1.AEAGVDAHE1.SYVEMHGTGTQAGDG1.EMTSVTNAFAppnrrrtpdek1.f1.gaikanvgiigeaasgins1.akv1.mmfkknaipanvgikgvmnesfpkd1.gernvhIP1.KQVEFPRNGDAPRK1.F1.NNFSAAGGNTS1.1.1.EDGPVRKPSTVKDPRST1.PITvTARS1.AS1.KRNIDN1.ksyi-Sktpeasits1.sytstarriqhnyriafpatdinkvsea1.daqikdsyspvaitatrvaFCFTGQGsqytg1.gqk1.yqd1.psfkadidq1.nq1.aeshn1.psf1.e1.fdgtdvat1.spvkvq1.gtaciQva1.srmweSwgikpsavighs1.geyaa1.hvagvisasdmvy1.vgrrzxe1.1.vkdctphthgm1.avkasvDairna1.gskMrEMacingpeetv1.cgsseivtaanev匚rsGKSTK1.NVPFAEHSAQVDP1.1.ESFRTIASSVSFKKPAVPV1.SP1.SGD1.ITDVGV1.GPEY1.AKHARETVNrSQA1.ESGQKAK1.FDGKTAW1.E1.GAHPvC1.gmvkgsveTTATAPS.rrgedawkti.ansmcai.h.Agvyinfdeyhrafndaqe1.1.h1.ptysfddkKYw1.dyhnnwt1.TKGEAPyPTKA1.EAAPHiAAAPVERTYKK1.SDSCQKHDEEFSANAGRV1.vQSDVCQPN1.RrvamghqvnDta1.cpss1.yfdmamtvadyiyka1.rpsapkigynvzvhmevpkpfiiknvzxppegqh1.q1.eAhad1.eegvAK1.VFRSVTPNGKQ1.VVHAHCTVKFEDINTWQEGwENISFMvKSQ1.EN1.QRKTRTQEAHVigrg1.ayk1.fKVrVNYAKPYRGMEEu1.DNQTTEATASVHFQTKPEDGEFFFPAYWCDSMAH1.GGFIVNATd1.cdsdnyvSvshgwgsikvakemszxnkkyrnyvrmvekpgnvtqgdvyifdgdeiiavvga1.kfqkiPRKV1.NTF1.PPQKAGSAvSApVAPPAAARPA1.KQAPRINTQAQPAKAVPKQVTvAApTPKAAPKKAD1.KKPAgptiitrvmHiiaaetdvdsse1.vdqaafen1.gv)S1.ms1.tis.xrfree1.dmdisst1.td11>tvgemkkfFSQYDGEVGtpeqddsdsdsetsgdastpmsevgtpmtipssavsesgkfd1.dtaapasgevsiarqivatemgveiapvavpapsnrRpsspq1.dkvnek1.nasrsadiskypazvtsv1.1.qgnpkizxtkkiff1.pdgsgsatsyvsipniGSDVCAFG1.NCPFMKSPEQWSCGIE1.CAMVY1.KE1.KRRKpTGPYn(iHhAYAVAQA1.1.ANNEEvek1.1.1.1.dSpcpvn1.dp1.p.Xr1.hiffneig1.1.gtgdpsktpkw1.1.phfsaairs1.sdyqpqptikpvktyaIWCREGVAGNPGDPRPPPAEDEDPAPMKW1.1.NHRTDFrDNGWgk1.cgvenmkygvmgghhfsmmKppiivsifdysivDdeanmrqaed1.gq1.ikeg1.sm> ICercosporanicotiaweMedgaqmrvvafgdqtydcseavsq1.1.rvrddaivvdf1.erapav1.kae1.ar1.sseqqeEtprfat1.ae1.VPRYRAGT1.NPAVSQA1.TCIAQ1.G1.FIRQHSSGQEAYPTAHDSC1.TGVCTGA1.；TAVAVGSASSvta1.vp1.A1.HIvavavr1.garaweigsc1.adarrgangryaswIsAVGGiSPQD1.QDRiSAYrAEQA1.ASVSVPY1.SAavgpgqssvsaapvi1.daf1.st1.1.rp1.tttr1.pitzxpyhaph1.ftakdvqhvtdc1.ppsezxWFTVRIP1.ISFSRDEAVSRGASFPAAMSEAVRr)C1.1.RP1.A1.DRMAVSrrNHARD1.GKDSV1.PSP1.A1.SFSDK1.GPQVNSH1.PGAKApTPE1.TSK31PSA1.GAEQQPMAKSP1.A1.1.AASGRFPQSSSMDQFWDV1.INGVDTHe1.vpptrWnaathvsedpkaknvsgtgfgcw1.heagefdaayfnmspreapqvdpaqr1.a1.1.tatea1.eqagvvpnrTsstqknrvgvwygatsndwmetnsaqnvdtyfipggnrafipgrvnyfhkfsgpsytidHMAcNA1.wRGEvDTAivGGTNv1.TNpDMTAG1.DAG1.IF1.SRSGNCKTfDDEADGYCRGEAvvt1.i1.kr1.pdAqadkdpiqasi1.giatnhsaezxzxsitrphagaqqd1.fqqv1.tetg1.tandisvcemhgtgtqagdsgetTSVVET1.AP1.NRSGSAVKnp1.Y1.GAVKSNVGHAESAAGVSS1.AKI1.1.M1.KHSK1.PPHVGIKTK1.NHRI.PD1.aarnthiarsevpwprpkngkrrv1.1.nnfsaaggntc1.v1.edapepedsqevdprehhiva1.sakpdSmvnnijxmitwidkhsgds1.at1.ijQI.SYTTrARRVHHRHRAVATGTD1.1.QiRSS1.QEQ1.DRRVSGERS1PHPPNGPSFV1.AFTGQGSAFAGMGVD1.YKRFASFRSDIARYDQICEGMS1.PSIKAMFEDEKVfstaspt1.Qq1.thvcfqma1.yr1.wks1.gvqakavvghs1.geyaa1.yaagv1.sqsdt1.y1.vgrr1Aq1.Mekh1.sqgtiiam1.avrakeeaivaaidgppgeayefscrxgeqrnv1.ggtvaqiqaakaa1.eakkircqy1.dTPNbXFHTGQVDpi1.pe1.1.qvaaacsiqdpqipvispaygkvirsakdfqpeyfthhcrssvnmvda1.qsaveeg1.1.dknvIG1.EIGPGPVvTQFVKEAVGTrMQTFASINKDKDTWQ1.MTQA1.AKFY1.AGASVEwSRYHEdfpgaqkv1.e1.paygwa1.knyv1.qyVndws1.rkgdpavvVaasn1.e1.sssihkViTNTiTANSDGE1.vVDad1.sredi.hpMvqghqvygvp1.ctpsvyadia1.t1.geyirqvikpgevaqtsvevaemniqsa1.vanntg