RAW2647小鼠巨噬细胞protocol.docx
RW264.7细胞株的相关信息RAW2e4.7nU$431.0My:ATCCMtte及太费图:5W>*!H三H索取报价VAccmw<三y<:*ItS人:ATCC«啪由工电懦:1800638659T如伴:onafle<vcom*址:395OfSttfPoifrtBMI.Su>ei8三>e三tt5JUff0TIi随计划让您仍为加心««|X«DSign徜onsRAW网7Dpo¾4or¾WCRwchM,Bcate<1.ml2S118d*oenXeSUmA8«PrwgalwSerumGroWr)XhgMProperties.03nTAZ5XiaV3monocyle*nacfopha99XOrPok>WShjmcBA1.BkIbwcsuitesDtseMe:Abe<tonmunneleukemiavrus11edtumorCeiIyp:mtcroaoe.AbelsonrrMrbewuemsirttwr»/TrnfrdhrsccymS*WonIo9MTAme11noneeabove,otherATCCanorrwW<vw114sEbeRWMforMetansferofPmMtFo<n8T¾ATCCmrenAfrronepurchasingATCm秋ggmwyrgwns<?forowningtheerme;P¼ACtoCkFwfQVrmanonrg*dngZwcifkWMrRmeftSforSNprrwnItoVWlocalsBoloaiCdiresponseApplkauonsYansracaonhotSoIUf8CeHuUfProductsReccplorsAntanEnxeseion.Me.CarWWcomplemr<(C3)1207H-MadultmaACommentsSubculhJfingReuiedProductsFor-Profit:$?Non-Profit:12Q"1JAddtoCaitThislinewasWUmh",gaWfWn<Jc6AhelsonmurtnejgmuWUftTMy第。n0阿2IeWSUCCgImmuncfllobulin<sA9>).la0>>andThyI2(Tfrr12)ThislinedoesnotsecretedetectablevrsPartCH3andnnegate讷me×Coeft)rmonassayTheCeU*iOQgIOsnr伯Ganoaooc¼<osew»>beadsandrmosanTheyamcapablef4ntb0d/dep«fM3er<giOfShMOeMg¼sWtumorelAgffK1.PSorPPDtealmentftx2daytt3rrMateshrwsoffMroc4wbutno!tumorcelUrgeisD3Oco<munca!edInFg2007OrJanetWKirtey.ncsr»»w11wcnofnfe(8usecotropicMU1.Va<9",觥皿r110te11)cMtotcMo1911vl*vhelperwususedintheCngmaWU$InOCUUmTocellsKsocipresspoFoWu1.V1guhsif19&basedonZmou«pa99冷hisk*rctney11slocks(Put*d1S1775OCATCCc<M11tetgrowthIiwdMNncThebasemediumfcf9HscelllineaATCC-VmuIatodDb4ccoMc<3iodEe'¾MeaUmtCataBNo30-2002Tomatethecompletegr*1medum.ac<3teWI0*1ngcomponentstoretswmeourn倘#em½africorcnv1ono11O%AtmospticfQ:ar,95%,cart>311dlo)do<C02),5%UnpeU*37.(TCPTotSuuuIMGft3f980呷90”卬WngFora75cm2ft*sk,rem(wtbut!Omlculturemedium(adustmourrtaccorntftorctercuturevssels)D8c*wcesfromtneflasksubstrate*ia(蝴Mfaperaspiratend*»appwiea04sofme(剖suspensioninto11o,*cubJf8v*M½SubcuIlKMtKmMio:ANbCUESr10011.3o1.6ne<omrrn<dIMtalmRmiawMRgploceOfadomumsery2»3darr$freezermum:CcmpUOeQrm0umsupplemcrtcdwl6%C)DMSO袁"十<*itM<N:quidMrOoenvaporphaseRecommended11wum(VtOhouttheaddrtonaiSUPQtemonnCfMrUmdscnt4<3underATCCMeUrn)ATCC302002faomfw3MServnATCC30-20201135Raqp*iP.NakorcIArUDotffdependantMlingofo11trc<WandtumortargetsC7mrop4rolledcclIretenhncemefrtbyPPDand1.PSJlmn011199>951.1977PubMW8940311207R<hk*WC.etFuncnonalmcro<M99CS皿GtQnfomwdbyAbgisoniauk«niaMru$Coll1526127,1978PubWed21219832443OeMnger1.C.MaR9UtaeonOfinducibiertrtco>desr<asee«pre$ionWM就jePunnorecoplorsandcakiumJBicrtChem271337-342.1926PufcWd855058332466HambleionJ.elAxtMflonfc-Junrerminmkna«inOtclenalIipopoifSMcMnde-SlrrMatedEJKepggggProcNsaKWSdUSA932774-277,W,PubMed861011632553:YWOrGAtelalIdontncMionofancvlGTPt*.r»enducbtfexprosedGTP*,tna3ccumU3tesintegpccWoM而5JCrlefn27i203992043.1996PUb"的"02"6DUlbcco,sModifiedEagle'sMedium(DMEMMATCCg30-2002nATCCNinnbec30-2002OuatrtNy:500m1.Stoiage:Slommediumat2,Cto8,Cinthedarkwenin5eApplications:DUgeeo3MoajftedEagle'sfea>um(DM£M)motetocontain4rrM1.-gM&mtne.4500rrgf1.gfcose,1rrMSOdiumpyruvalefsdiXOmgf1.sodiumbicat×m那.Formulation:Fam3灰IOnProductFormat:500m1.Comments:NOTE:This/educedfevHofsdfumbica,bomg(NHCO3,1.5gf1.)isintendedforUSein5%CO2inair.AddHionsisodiumbicarbon*QmayberequitedforuseinncbtorscontainingigherpercentagesofCO2SubculturingProcedureSubculturesarepreparedbyscrapingFora75cm2flask,removeallbut10mlculturemedium(adjustamountaccordinglyforotherculturevessels).Dislodgecellsfromtheflasksubstratewithacellscraper;aspirateandaddappropriatealiquotsofthecellsuspensionintonevculturevessels.SubcultjvationRatio:1:3to1:6MediumRenewalReplaceoraddmediumevery2to3days.CompleteGrowthMediumThebasemediumforthiscelllineisATCC-formulatedDulbecco'sModifiedEagle'sMedium.CatalogNo.30-2002.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%Thismediumisformulatedforusewitha5%CO2inairatmosphere.(StandardDMEMformulationscontain3.7g/1.sodiumbicarbonateanda10%CO2inairatmosphereisthenrecommended).ATCCtestedfetalbovineserumisavailableasATCCCatalogNo.30-2020andATCCCatalogNo.30-2021(100ml).CryoprotectantMediumCompletegrowthmediumdescribedabovesupplementedwith5%(vv)DMSO.ATCC:美国模式培育物集存库(AmeriCantypeculturecollection)的简写收到冷冻管细胞的处理方式1.收到细胞株包袱时,请检查细胞株冷冻笆是含有解冻情形.心清勺上通知.细胞株请尽速起先培杼,或马上冷冻保存(置干-70C,隔夜后,移到液氮)。2.收到T25flask细胞的处理方式于寄送过程中,为避开起泡造成细咆脱落死亡,T25flask均加满培1. 1.检查flask外观,并于显微境下视察细胞生长状况和有无污染现象,若有任何问遨.不要打开装子,请马上通知细胞试脸室.2. 2.将原财之T25flask静次于379,5%CQ2培Ff箱中,使细胞回温至37C,并让运输过程中少数脱落的细胞可再附着生长.2.3.隔天后,于无菌操作箱内取出flask内之培育基.(取出之培育基可以再运用),仅留妁5-10l培闫基于flask内.依-般培H方式再将细胞谀入培育和中,或细胞已长满盘,则将细胞做传代培育,RAW