痂囊腔菌属PKS基因特性分析.docx

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1、Characterizationofpo1.ykctidcsynthasegenesfromE1.sinoespp.和囊腔MPKS嘱因特性分析目的：得到花生疮痂病的的PKS域因，明确其PKS的结构域，证明由素的合成受到PKS的网控.方法：I使用简并引物对其胡君合iKS保守区进行扩增，通过染色体步格，泅得其全长.2对荻得的全长翻译后进行宽基酸的进化树分析.确定其种类3对基因表达进行研究讨论：Materia1.andmethodsFunga1.strainsandgrowthconditions尾胞(Genebanktiff).柑橘(Genebimk己有，他葡,花生Iso1.ationofthek

2、etovnthase(KS)domainusingdegenerateprimers采用简并引物扩增PKS的KS保守区域.步骤方法Identificationofpartia1.PKSsequencesKS保守区域熨基取序列对比(尾他.柑橘.匍葡,花生.黄曲褥毒索,爆刀菌素)Genomewa1.king获得权因全长，标记各个PKS域分布及与其他相似的比较SAPKSPhy1.ogeneticana1.ysisofPKSsequences进化树选择不同类型的PKS基因类型1.2.3),选择还原鞭及非还原型.(参考2003)选择还原型和非还原型两种.Expressionana1.ysesofPKSg

3、enes测定各个域的表达量及整个基因的衣达量，明确该基因的表达是否与光照有相关性，或者存在其他相关联基因共同调节次生代谢产物.SAPKSEkafage1.gsigtssr1.vtayea1.eesgyvpnrtpstk1.iirigtfygqtsddwreinaaenVdtyfitggvrafakirixyyfkfsgpsfsv,Baaiqi.actsi.wagkdtacagg1.nv1.tnpdifsg1.skgqf1.sktgscktydndadgycrgdgcgsvi1.kryedaiadkdni1.Gci1.gaatnhsaeavsitiiphagaqef1.fkkv1.adagida

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